KMID : 0545119960060060381
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Journal of Microbiology and Biotechnology 1996 Volume.6 No. 6 p.381 ~ p.385
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A Plasmid of Lactococcus lactis subsp. lactis ML8 Linked with Lactose Metabolism and Extracellular Proteinase
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Lee Jong-Hoon
LEE HYONG-JOO
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Abstract
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Three distinct plasmids, with approximate molecular weight of 1, 4.5, and 33 megadaltons, were found in Lactococcus lactis subsp. lactis (L. lactis) ML8. Slow acid-producing mutants of L. lactis ML8, isolated by plasmid curing with acriflavine treatment, lacked the 33-megadalton plasmids. The plasmidcured mutant showed lactose-negative (Lac) characteristics and the alteration of extracellular peoteinase pattern. The possible involvement of extracellular peoteinase with the 33-megadalton plasmid is highlighted in this research.
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